CD35 (CR1) Mouse Monoclonal Antibody [Clone ID: E11]
CAT#: SM1097P
CD35 (CR1) mouse monoclonal antibody, clone E11, Purified
Size: 100 ug
Conjugation: FITC
Specifications
Product Data | |
Clone Name | E11 |
Applications | FC, IHC, WB |
Recommended Dilution | Flow Cytometry: Use 10 µl of 1/50-1/200 diluted antibody to label 106 cells or 100 µl whole blood. Immunohistochemistry on Frozen Section: 1-10 µg/ml. Immunohistochemistry on Paraffin Section: 1-10 µg/ml. Requires antigen retrieval using heat treatment prior to staining of Paraffin sections. Sodium citrate buffer pH6.0 is recommended for this purpose. Recommended Positive Control: Human Tonsil. Western Blotting (Ref.3). |
Reactivities | Human, Monkey, Baboon |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Human acute monocytic leukaemia cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line. |
Specificity | This antibody is specific for CD35, a single chain cell surface glycoprotein which exists in four allotypic forms (A,B,C,D) of 190kD, 220kD, 160kD and 250kD respectively. |
Formulation | PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein A |
Conjugation | Unconjugated |
Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Gene Name | complement component 3b/4b receptor 1 (Knops blood group) |
Database Link | |
Background | CD35 is a single chain cell surface glycoprotein which exists in four allotypic forms (A,B,C,D) of 190kD, 220kD, 160kD and 250kD respectively. The molecule is expressed by granulocytes, monocytes, B cells and some T cells. Functionally CD35 acts as a receptor for the C3b component of complement. |
Synonyms | Complement receptor type 1, CR1, C3b/C4b receptor |
Reference Data |
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