Cyanine Dyes Sheep Polyclonal Antibody

CAT#: R1460F

Cyanine Dyes sheep polyclonal antibody, FITC

Specifications

Product Data

• Applications: FC, IF
• Recommended Dilution: Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: A mixture of Cy3 and Cy5 conjugated KLH
• Specificity: This product was prepared from monospecific antiserum by Immunoaffinity chromatography using a cyanine BSA conjugate coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum and cyanine conjugated IgG.
  Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analog is unknown.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide as preservative and 10 mg/ml Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.
  Label: FITC
  State: Lyophilized purified Ig fraction
  Label: Fluorescein isothiocyanate (MW 390 daltons)
  Absorption emission: 495 nm / 528 nm
  Molar radio: 4.0 moles FITC per mole of Sheep IgG
• Reconstitution Method: Restore with 1.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Conjugation: FITC
• Storage: Store vial at 2-8°C prior to restoration. For extended storage mix with glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.