DAO Sheep Polyclonal Antibody

CAT#: R1056HRPS

DAO sheep polyclonal antibody, HRP, Purified

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western blot: 1/500-1/5,000.
  Immunoprecipitation: 1/100.
  ELISA: 1/5,000-1/20,000.
  This product has been assayed against 1.0 µg of D-amino-acid oxidase [pig kidney] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/12,000 to 1:60,000 of the reconstitution concentration is suggested.
• Reactivities: Porcine
• Host: Sheep
• Clonality: Polyclonal
• Immunogen: D-amino-acid oxidase from porcine kidney
• Specificity: This antibody detects porcine D-amino-acid oxidase.
  Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-sheep serum as well as purified and partially purified D-amino-acid oxidase [pig kidney].
• Formulation: 0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2
  Label: HRP
  State: Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free)
  Preservative: 0.01% (w/v) Gentamicin sulfate (Do NOT add Sodium azide!)
  Label: Horseradish peroxidase
• Reconstitution Method: Restore with 0.1 ml of deionized water (or equivalent).
• Concentration: lot specific
• Purification: Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
• Conjugation: HRP
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P00371
• Background: The DAO gene encodes the peroxisomal enzyme D amino acid oxidase. The enzyme is a flavoprotein which uses flavin adenine dinucleotide (FAD) as its prosthetic group. Its substrates include a wide variety of D amino acids, but it is inactive on the naturally occurring L amino acids. Its biological function is not known; it has been suggested that it is involved in acid base balance in the kidney or it could act as a detoxifying agent which removes D-amino acids accumulated during aging, or it may be a fossil enzyme without a current function.
• Synonyms: DAO, DAMOX, DAAO, OXDA, DAO1