TIE2 (TEK) Mouse Monoclonal Antibody [Clone ID: Cl.16]

CAT#: DM3511B

TIE2 (TEK) mouse monoclonal antibody, clone Cl.16, Biotin

Specifications

Product Data

• Clone Name: Cl.16
• Applications: ELISA, FC, WB
• Recommended Dilution: Western Blot.
  ELISA: 1-2 µg/ml.
  FACS analysis and cell sorting: 2-5 µg/ml.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human soluble CD202b / TIE-2 protein
• Specificity: The unconjugated monoclonal antibody will detect native human TIE-2 /TEK in ELISA experiments and on the surface of different human cell types.
• Formulation: PBS
  Label: Biotin
  State: Lyophilised purified IgG fraction from culture supernatant
  Stabilizer: 50x BSA
  Preservative: 0.02% Sodium Azide
• Reconstitution Method: Restore to a concentration of 0.1-1.0 mg/ml with sterile water. Centrifuge vial prior to opening
• Purification: Protein G Chromatography
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: TEK receptor tyrosine kinase
• Database Link:
  Entrez Gene 7010 Human
  UniProt ID Q02763
• Background: TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin like loops separated by three epidermal growth factor like repeats that are connected to three fibronectin type III like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations; the TIE2 signaling pathway appears to be critical for endothelial cell smooth muscle cell communication in venous morphogenesis.
• Synonyms: TIE2, TIE-2, Angiopoietin-1 receptor, p140 TEK