Cytokeratin 10 (KRT10) Mouse Monoclonal Antibody [Clone ID: DE-K10]

CAT#: DM055

Cytokeratin 10 (KRT10) mouse monoclonal antibody, clone DE-K10, Supernatant

Specifications

Product Data

• Clone Name: DE-K10
• Applications: IHC, WB
• Recommended Dilution: Western Blot.
  Cell Suspensions.
  Immunohistochemistry on Frozen Sections: 1/5-1/10 (Fixation with acetone for 5 min. at -20°C is recommended).
  Immunohistochemistry on Paraffin Sections: 1/100 (Fixation with neutral formalin and a pretreatment with pronase or trypsin is also recommended).
  Recommended Positive Control: Squamous cell carcinoma, epidermis.
  Incubation Time: 1 h at room temperature.
• Reactivities: Canine, Feline, Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Cytoskeletal preparation from Human epidermis.
• Specificity: This antibody specifically recognizes the 56.6 kDa Keratin K10 (formerly also designated Cytokeratin 10) polypeptide in squamous cells and carcinomas from e.g. epidermis, lung, bladder, cervix, oesophagus.
• Formulation: 0.15M PBS
  State: Supernatant
  State: Liquid Tissue Culture Supernatant
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: keratin 10
• Database Link:
  Entrez Gene 3858 Human
  UniProt ID P13645
• Background: Cytokeratin 10 is a heterotetramer of two type I and two type II keratins. Cytokeratin 10 is generally associated with keratin 1. It is seen in all suprabasal cell layers including stratum corneum. A number of alleles are known that mainly differ in the Gly-rich region (positions 490-560). Defects in cytokeratin 10 are a cause of epidermolytic hyperkeratosis (EHK), also known as bullous congenital ichthyosiform erythroderma (BCIE) or bullous erythroderma ichthyosiformis congenita of Brocq. EHK is an hereditary skin disorder characterized by blistering and a marked thickening of the stratum corneum. At birth, affected individuals usually present with redness, blisters and superficial erosions due to cytolysis. Within a few weeks, the erythroderma and blister formation diminish and hyperkeratoses develop. Transmission is autosomal dominant, but most cases are sporadic. Defects in cytokeratin 10 are also a cause of annular epidermolytic ichthyosis (AEI), also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI resembles clinical and histologic features of both epidermolytic hyperkeratosis and ichthyosis bullosa of Siemens.
• Synonyms: Cytokeratin-10, CK10, CK-10, Keratin-10, KRT10, KPP, K10