TDP43 (TARDBP) Mouse Monoclonal Antibody [Clone ID: OTI1D1]

CAT#: CF814154

Carrier-free (BSA/glycerol-free) TARDBP mouse monoclonal antibody, clone OTI1D1

Product Images

Figure A, Western blot analysis of overexpressed lysates(15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC) , human TARDBP plasmid (RC210639, hTARDBP), mouse TARDBP plasmid (MR206534, mTARDBP) , rat TARDBP plasmid (RR209466, rTARDBP) using anti-TARDBP antibody TA814154(1:500). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:1000)

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  Western blot analysis of extracts (50ug per lane) from 3 cell lines lysates by using anti-TARDBP monoclonal antibody(TA814154, 1:500).

Specifications

Product Data

• Clone Name: OTI1D1
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment of Human TARDBP (NP_031401) produced in Ecoli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 44.7 kDa
• Gene Name: TAR DNA binding protein
• Database Link:
  NP_031401
  Entrez Gene 230908 MouseEntrez Gene 23435 Human
  UniProt ID Q13148
• Background: The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008]
• Synonyms: ALS10; TDP-43

Reference Data

• Protein Families: Transcription Factors