Y14 (RBM8A) Mouse Monoclonal Antibody [Clone ID: OTI10B5]

CAT#: CF812498

Carrier-free (BSA/glycerol-free) RBM8A mouse monoclonal antibody,clone OTI10B5

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY RBM8A (Cat# RC209770, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RBM8A (Cat# TA812498)(1:500). Positive lysates LY417520 (100ug) and LC417520 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 4 cell lines lysates by using anti-RBM8A monoclonal antibody (1:250).

Specifications

Product Data

• Clone Name: OTI10B5
• Applications: WB
• Recommended Dilution: WB 1:250~500
• Reactivities: Human, Rat, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 2-174 of human RBM8A (NP_005096) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 19.7 kDa
• Gene Name: RNA binding motif protein 8A
• Database Link:
  NP_005096
  Entrez Gene 60365 MouseEntrez Gene 295284 RatEntrez Gene 9939 Human
  UniProt ID Q9Y5S9
• Background: This gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. There are two alternate translation start codons with this gene, which result in two forms of the protein. An allele mutation and a low-frequency noncoding single-nucleotide polymorphism (SNP) in this gene cause thrombocytopenia-absent radius (TAR) syndrome. [provided by RefSeq, Jul 2013]
• Synonyms: BOV-1A; BOV-1B; BOV-1C; C1DELq21.1; DEL1q21.1; MDS014; RBM8; RBM8B; TAR; Y14; ZNRP; ZRNP1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Spliceosome