MLX Mouse Monoclonal Antibody [Clone ID: OTI1D11]

CAT#: CF810951

Carrier-free (BSA/glycerol-free) MLX mouse monoclonal antibody,clone OTI1D11

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MLX (Cat# RC201911, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MLX (Cat# TA810951)(1:500). Positive lysates LY404977 (100ug) and LC404977 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1D11
• Applications: WB
• Recommended Dilution: WB 1:500
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MLX (NP_937847) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 27.7 kDa
• Gene Name: MAX dimerization protein MLX
• Database Link:
  NP_937847
  Entrez Gene 21428 MouseEntrez Gene 360631 RatEntrez Gene 6945 Human
  UniProt ID Q9UH92
• Background: The product of this gene belongs to the family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. These factors form heterodimers with Mad proteins and play a role in proliferation, determination and differentiation. This gene product may act to diversify Mad family function by its restricted association with a subset of the Mad family of transcriptional repressors, namely, Mad1 and Mad4. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
• Synonyms: bHLHd13; MAD7; MXD7; TCFL4; TF4

Reference Data

• Protein Families: Druggable Genome, Transcription Factors