TCF12 Mouse Monoclonal Antibody [Clone ID: OTI1G10]

CAT#: CF809695

Carrier-free (BSA/glycerol-free) TCF12 mouse monoclonal antibody,clone OTI1G10

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TCF12 (RC220164, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TCF12 (1:2000). Positive lysates LY403720 (100ug) and LC403720 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1G10
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human TCF12 (NP_996919) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 75.7 kDa
• Gene Name: transcription factor 12
• Database Link:
  NP_996919
  Entrez Gene 21406 MouseEntrez Gene 25720 RatEntrez Gene 6938 Human
  UniProt ID Q99081
• Background: The protein encoded by this gene is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. This encoded protein is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq, Jul 2008]
• Synonyms: bHLHb20; CRS3; HEB; HsT17266; HTF4

Reference Data

• Protein Families: Druggable Genome, Transcription Factors