ARHGAP12 Mouse Monoclonal Antibody [Clone ID: OTI4A3]

CAT#: CF809263

Carrier-free (BSA/glycerol-free) ARHGAP12 mouse monoclonal antibody,clone OTI4A3

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY ARHGAP12 (Cat# RC215343, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARHGAP12 (Cat# TA809263)(1:2000). Positive lysates LY413135 (100ug) and LC413135 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI4A3
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 286-452 of human ARHGAP12 (NP_060757) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 96.1 kDa
• Gene Name: Rho GTPase activating protein 12
• Database Link:
  NP_060757
  Entrez Gene 75415 MouseEntrez Gene 307016 RatEntrez Gene 94134 Human
  UniProt ID Q8IWW6
• Background: This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein may be involved in suppressing tumor formation by regulating cell invasion and adhesion. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
• Synonyms: DKFZp779N2050; FLJ10971; FLJ20737; FLJ21785; FLJ45709