TRIM56 Mouse Monoclonal Antibody [Clone ID: OTI5A1]

CAT#: CF808895

Carrier-free (BSA/glycerol-free) TRIM56 mouse monoclonal antibody, clone OTI5A1 (formerly 5A1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY TRIM56 (Cat# RC200891, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM56 (Cat# TA808895)(1:2000). Positive lysates LY410625 (100ug) and LC410625 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-TRIM56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808895) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-TRIM56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808895) (1:150)

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  Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-TRIM56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808895) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-TRIM56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808895) (1:150)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-TRIM56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808895) (1:150)

• Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-TRIM56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808895) (1:150)

• Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-TRIM56 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min, TA808895) (1:150)

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Specifications

Product Data

• Clone Name: OTI5A1
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 311-554 of human TRIM56(NP_112223) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 81.3 kDa
• Gene Name: tripartite motif containing 56
• Database Link:
  NP_112223
  Entrez Gene 81844 Human
  UniProt ID Q9BRZ2
• Background: E3 ubiquitin-protein ligase that mediates 'Lys-63'-linked polyubiquitination of TMEM173/STING, thereby playing a key role in innate immunity. TMEM173/STING 'Lys-63'-linked ubiquitination activates the production of type I interferon IFN-beta following detection of pathogen- and host-derived double-stranded DNA. [UniProtKB/Swiss-Prot Function]
• Synonyms: RNF109