NDUFB11 Mouse Monoclonal Antibody [Clone ID: OTI12H10]

CAT#: CF807792

Carrier-free (BSA/glycerol-free) NDUFB11 mouse monoclonal antibody, clone OTI12H10 (formerly 12H10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDUFB11 (RC213937, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDUFB11 (1:2000). Positive lysates LY412795 (100ug) and LC412795 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI12H10
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 30-163 of human NDUFB11(NP_061929) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18.2 kDa
• Gene Name: NADH:ubiquinone oxidoreductase subunit B11
• Database Link:
  NP_061929
  Entrez Gene 54539 Human
  UniProt ID Q9NX14
• Background: NDUFB11 is a component of mitochondrial complex I. Complex I catalyzes the first step in the electron transport chain, the transfer of 2 electrons from NADH to ubiquinone, coupled to the translocation of 4 protons across the membrane (Carroll et al., 2002 [PubMed 12381726]). [supplied by OMIM, Feb 2009]
• Synonyms: CI-ESSS; ESSS; Np15; NP17.3; P17.3

Reference Data

• Protein Families: Transmembrane