Midkine (MDK) Mouse Monoclonal Antibody [Clone ID: OTI6C8]

CAT#: CF807679

Carrier-free (BSA/glycerol-free) MDK mouse monoclonal antibody, clone OTI6C8 (formerly 6C8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MDK (Cat# RC221818, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MDK (Cat# TA807679)(1:2000). Positive lysates LY425316 (100ug) and LC425316 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI6C8
• Applications: WB
• Recommended Dilution: WB 1:2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 21-143 of human MDK(NP_001012334) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 15.4 kDa
• Gene Name: midkine
• Database Link:
  NP_001012334
  Entrez Gene 17242 MouseEntrez Gene 81517 RatEntrez Gene 4192 Human
  UniProt ID P21741
• Background: This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul
• Synonyms: ARAP; MK; NEGF2

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane