SNX12 Mouse Monoclonal Antibody [Clone ID: OTI1B10]

CAT#: CF804639

Carrier-free (BSA/glycerol-free) Purified SNX12 mouse monoclonal antibody, clone OTI1B10 (formerly 1B10)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNX12 (RC224303, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNX12. Positive lysates LY415650 (100ug) and LC415650 (20ug) can be purchased separately from OriGene.

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SNX12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804639)

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  Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-SNX12 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, TA804639)

Specifications

Product Data

• Clone Name: OTI1B10
• Applications: IHC, WB
• Recommended Dilution: WB 1:2000, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human SNX12 (NP_037478) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 18.7 kDa
• Gene Name: sorting nexin 12
• Database Link:
  NP_037478
  Entrez Gene 55988 MouseEntrez Gene 363478 RatEntrez Gene 29934 Human
  UniProt ID Q9UMY4
• Synonyms: MGC118982; MGC118983