Mitofusin 1 (MFN1) Mouse Monoclonal Antibody [Clone ID: OTI4D11]

CAT#: CF802148

Carrier-free (BSA/glycerol-free) MFN1 mouse monoclonal antibody,clone OTI4D11

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MFN1 (Cat# RC207184, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MFN1 (Cat# TA802148). Positive lysates LY403252 (100ug) and LC403252 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 2 different cell lines by using anti-MFN1 monoclonal antibody (1:200).

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MFN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802148)

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  Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-MFN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, TA802148)

Specifications

Product Data

• Clone Name: OTI4D11
• Applications: IHC, WB
• Recommended Dilution: WB 1:200, IHC 1:150
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 209-469 of human MFN1 (NP_284941) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 84 kDa
• Gene Name: mitofusin 1
• Database Link:
  NP_284941
  Entrez Gene 67414 MouseEntrez Gene 192647 RatEntrez Gene 55669 Human
  UniProt ID Q8IWA4
• Background: The protein encoded by this gene is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). They are mitochondrial membrane proteins that interact with each other to facilitate mitochondrial targeting. [provided by RefSeq, Jul 2008]
• Synonyms: hfzo1; hfzo2