ING2 Mouse Monoclonal Antibody [Clone ID: OTI1E12]

CAT#: CF800642

Carrier-free (BSA/glycerol-free) ING2 mouse monoclonal antibody, clone OTI1E12 (formerly 1E12)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ING2 (RC202478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ING2. Positive lysates LY419856 (100ug) and LC419856 (20ug) can be purchased separately from OriGene.

• 

  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-ING2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Specifications

Product Data

• Clone Name: OTI1E12
• Applications: WB
• Recommended Dilution: WB 1:500~2000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human ING2 (NP_001555) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.6 kDa
• Gene Name: inhibitor of growth family member 2
• Database Link:
  NP_001555
  Entrez Gene 69260 MouseEntrez Gene 290744 RatEntrez Gene 3622 Human
  UniProt ID Q9H160
• Background: This gene is a member of the inhibitor of growth (ING) family. Members of the ING family associate with and modulate the activity of histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes and function in DNA repair and apoptosis. [provided by RefSeq, Jul 2008]
• Synonyms: ING1L; p33ING2

Reference Data

• Protein Families: Druggable Genome, Transcription Factors