Meis homeobox 3 (MEIS3) Mouse Monoclonal Antibody [Clone ID: OTI3A7]

CAT#: CF800267

Carrier-free (BSA/glycerol-free) MEIS3 mouse monoclonal antibody, clone OTI3A7 (formerly 3A7)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MEIS3 (RC206100, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MEIS3. Positive lysates LY412624 (100ug) and LC412624 (20ug) can be purchased separately from OriGene.

• 

  Anti-MEIS3 mouse monoclonal antibody (TA800267) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MEIS3 (RC206100).

• 

  HEK293T cells transfected with either RC206100 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MEIS3 antibody (TA800267), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI3A7
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-261 of human MEIS3 (NP_064545) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 46 kDa
• Gene Name: Meis homeobox 3
• Database Link:
  NP_064545
  Entrez Gene 17537 MouseEntrez Gene 56917 Human
  UniProt ID Q99687
• Synonyms: MRG2