NYREN18 (NUB1) Mouse Monoclonal Antibody [Clone ID: OTI4H2]

CAT#: CF800188

Carrier-free (BSA/glycerol-free) NUB1 mouse monoclonal antibody, clone OTI4H2 (formerly 4H2)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY NUB1 (Cat# RC208098, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUB1(Cat# TA800188). Positive lysates LY414176 (100ug) and LC414176 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-NUB1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

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  Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800188)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800188)

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  Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA800188)

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  Anti-NUB1 mouse monoclonal antibody (TA800188) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUB1 (RC208098).

• HEK293T cells transfected with either RC208098 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NUB1 antibody (TA800188), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI4H2
• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
• Reactivities: Human, Dog, Rat, Monkey, Mouse
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-326 of human NUB1 (NP_057202) produced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 68.9 kDa
• Gene Name: negative regulator of ubiquitin like proteins 1
• Database Link:
  NP_057202
  Entrez Gene 53312 MouseEntrez Gene 296731 RatEntrez Gene 475542 DogEntrez Gene 715231 MonkeyEntrez Gene 51667 Human
  UniProt ID Q9Y5A7
• Background: NUB1 interacts with and negatively regulates NEDD8 (MIM 603171), a ubiquitin-like protein that covalently conjugates to cullin (see MIM 603134) family members. [supplied by OMIM]
• Synonyms: BS4; NUB1L; NYREN18

Reference Data

• Protein Families: Druggable Genome