IRF5 Mouse Monoclonal Antibody [Clone ID: OTI1B8]

CAT#: CF507251

Carrier-free (BSA/glycerol-free) IRF5 mouse monoclonal antibody, clone OTI1B8 (formerly 1B8)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IRF5 (RC200458, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IRF5. Positive lysates LY403185 (100ug) and LC403185 (20ug) can be purchased separately from OriGene.

Specifications

Product Data

• Clone Name: OTI1B8
• Applications: WB
• Recommended Dilution: WB 1:4000
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human IRF5(NP_116032) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 55.9 kDa
• Gene Name: interferon regulatory factor 5
• Database Link:
  NP_116032
  Entrez Gene 27056 MouseEntrez Gene 296953 RatEntrez Gene 3663 Human
  UniProt ID Q13568
• Background: This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Multiple transcript variants encoding different isoforms have been found for this gene, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq, Mar 2010]
• Synonyms: SLEB10

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Toll-like receptor signaling pathway