NIPSNAP2 Mouse Monoclonal Antibody [Clone ID: OTI1D6]

CAT#: CF505585

Carrier-free (BSA/glycerol-free) GBAS mouse monoclonal antibody, clone OTI1D6 (formerly 1D6)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBAS (RC205027, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBAS. Positive lysates LY400571 (100ug) and LC400571 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GBAS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Anti-GBAS mouse monoclonal antibody (TA505585) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GBAS (RC205027).

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  Immunofluorescent staining of HeLa cells using anti-GBAS mouse monoclonal antibody (TA505585).

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  HEK293T cells transfected with either RC205027 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GBAS antibody (TA505585), and then analyzed by flow cytometry.

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Specifications

Product Data

• Clone Name: OTI1D6
• Applications: FC, IF, WB
• Recommended Dilution: WB: 1:200-1:1000, IF: 1:100, FLOW: 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human GBAS(NP_001474) proeduced in E.coli.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33.6 kDa
• Gene Name: nipsnap homolog 2
• Database Link:
  NP_001474
  Entrez Gene 2631 Human
  UniProt ID O75323
• Background: This gene encodes a member of the NipSnap family of proteins that may be involved in vesicular transport. The encoded protein is localized to mitochondria and plays a role in oxidative phosphorylation. A pseudogene of this gene is located on the long arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2011]
• Synonyms: NIPSNAP2