katanin p80 (KATNB1) Mouse Monoclonal Antibody [Clone ID: OTI5E1]

CAT#: CF503814

Carrier-free (BSA/glycerol-free) KATNB1 mouse monoclonal antibody, clone OTI5E1 (formerly 5E1)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KATNB1 (RC201852, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KATNB1. Positive lysates LY417004 (100ug) and LC417004 (20ug) can be purchased separately from OriGene.

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  Anti-KATNB1 mouse monoclonal antibody (TA503814) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KATNB1 (RC201852).

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  HEK293T cells transfected with either RC201852 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-KATNB1 antibody (TA503814), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI5E1
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human KATNB1(NP_005877) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 72.2 kDa
• Gene Name: katanin regulatory subunit B1
• Database Link:
  NP_005877
  Entrez Gene 10300 Human
  UniProt ID Q9BVA0
• Background: Microtubules, polymers of alpha and beta tubulin subunits, form the mitotic spindle of a dividing cell and help to organize membranous organelles during interphase. Katanin is a heterodimer that consists of a 60 kDa ATPase (p60 subunit A 1) and an 80 kDa accessory protein (p80 subunit B 1). The p60 subunit acts to sever and disassemble microtubules, while the p80 subunit targets the enzyme to the centrosome. Katanin is a member of the AAA family of ATPases. [provided by RefSeq]
• Synonyms: KAT; LIS6