EIF4E Mouse Monoclonal Antibody [Clone ID: OTI6C11]
CAT#: CF502993
Carrier-free (BSA/glycerol-free) EIF4E mouse monoclonal antibody, clone OTI6C11 (formerly 6C11)
Formulation: Standard
Specifications
Product Data | |
Clone Name | OTI6C11 |
Applications | FC, WB |
Recommended Dilution | WB 1:500~2000, FLOW 1:100 |
Reactivities | Human, Mouse, Rat |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Full length human recombinant protein of human EIF4E (NP_001959) produced in HEK293T cell. |
Formulation | Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose) |
Reconstitution Method | For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific) |
Purification | Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) |
Conjugation | Unconjugated |
Storage | Store at -20°C as received. |
Stability | Stable for 12 months from date of receipt. |
Predicted Protein Size | 24.9 kDa |
Gene Name | eukaryotic translation initiation factor 4E |
Database Link | |
Background | All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]). [supplied by OMIM] |
Synonyms | AUTS19; CBP; eIF-4E; EIF4E1; EIF4EL1; EIF4F |
Reference Data | |
Protein Pathways | Insulin signaling pathway, mTOR signaling pathway |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Antibody Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
TA502993 | EIF4E mouse monoclonal antibody, clone OTI6C11 (formerly 6C11) |
USD 224.00 |
|
TA502993AM | EIF4E mouse monoclonal antibody, clone OTI6C11 (formerly 6C11), Biotinylated |
USD 509.00 |
|
TA502993BM | EIF4E mouse monoclonal antibody, clone OTI6C11 (formerly 6C11), HRP conjugated |
USD 509.00 |
|
TA502993S | EIF4E mouse monoclonal antibody, clone OTI6C11 (formerly 6C11) |
USD 200.00 |
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