MRAS Mouse Monoclonal Antibody [Clone ID: OTI3C4]

CAT#: CF502864

Carrier-free (BSA/glycerol-free) MRAS mouse monoclonal antibody, clone OTI3C4 (formerly 3C4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY MRAS (Cat# RC212259, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MRAS(Cat# TA502864). Positive lysates LY415896 (100ug) and LC415896 (20ug) can be purchased separately from OriGene.

• 

  Anti-MRAS mouse monoclonal antibody (TA502864) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MRAS (RC212259 ).

• 

  Flow cytometric Analysis of Jurkat cells, using anti-MRAS antibody (TA502864), (Red), compared to a nonspecific negative control antibody, (Blue).

Specifications

Product Data

• Clone Name: OTI3C4
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human MRAS (NP_036351) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 23.7 kDa
• Gene Name: muscle RAS oncogene homolog
• Database Link:
  NP_036351
  Entrez Gene 22808 Human
  UniProt ID O14807
• Background: Members of the RAS superfamily of GTP-binding proteins, which includes MRAS, are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors. [supplied by OMIM]
• Synonyms: M-RAs; R-RAS3; RRAS3

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: MAPK signaling pathway, Regulation of actin cytoskeleton, Tight junction