CAPZA1 Mouse Monoclonal Antibody [Clone ID: OTI2G4]

CAT#: CF501124

Carrier-free (BSA/glycerol-free) CAPZA1 mouse monoclonal antibody, clone OTI2G4 (formerly 2G4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY CAPZA1 (Cat# RC201642, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CAPZA1(Cat# TA501124). Positive lysates LY401848 (100ug) and LC401848 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-CAPZA1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).

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  Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-CAPZA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501124, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-CAPZA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501124, Dilution 1:50)

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  Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-CAPZA1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, TA501124, Dilution 1:50)

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  Flow cytometric Analysis of Jurkat cells, using anti-CAPZA1 antibody (TA501124), (Red), compared to a nonspecific negative control antibody, (Blue) (1:100).

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Specifications

Product Data

• Clone Name: OTI2G4
• Applications: FC, IHC, WB
• Recommended Dilution: WB 1:500, IHC 1:50, Flow 1:100
• Reactivities: Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human CAPZA1(NP_006126) produced in HEK293T cell.
• Formulation: Lyophilized powder (original buffer 1X PBS, pH 7.3, 8% trehalose)
• Reconstitution Method: For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. (OriGene recommends Zeba Spin Desalting Columns, 7KMWCO from Thermo Scientific)
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 32.7 kDa
• Gene Name: capping actin protein of muscle Z-line subunit alpha 1
• Database Link:
  NP_006126
  Entrez Gene 691149 RatEntrez Gene 829 Human
  UniProt ID P52907
• Background: CAPZA1 is a member of the F-actin capping protein alpha subunit family. This gene encodes the alpha subunit of the barbed-end actin binding protein. The protein regulates growth of the actin filament by capping the barbed end of growing actin filaments.
• Synonyms: CAPPA1; CAPZ; CAZ1