Collagen I (COL1A1) Rabbit Polyclonal Antibody
CAT#: BP8028S
Rabbit Polyclonal Anti-COL1A1 Antibody, Purified
BP8028S is a replacement of BP8028.
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Other products for "Collagen I"
Specifications
Product Data | |
Applications | ELISA, IF, IHC, R, WB |
Recommended Dilution | RIA. ELISA: 1/200 (OD ≥ 500). Immunflourescence Assays: 1/80. Immunohistochemistry on Paraffin Sections: 1/200-1/600. Pretreatment: After de-waxing the tissue slices they are treated with 0.2% Hyaluronidase (app. 300 U/mg) in TBS 15 min at 37°C. There after non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: 60 min at RT or 2-8°C over night. Positive Control: Human skin. |
Reactivities | Human |
Host | Rabbit |
Isotype | IgG |
Clonality | Polyclonal |
Immunogen | Purified Collagen type I from Human skin. |
Specificity | Human Collagen type I: 100% Collagen type II and IV: < 0.1% Collagen type III and V: < 1.0% Human Fibronectin: < 0.1% Cross-reactivity with cattle <1.0%, rat and chicken < 0.1% (RIA at a 1:500 dilution) |
Formulation | Phosphate buffered solution, no BSA and preservative added. State: Purified State: Lyophilized purified Ig fraction |
Reconstitution Method | Restore with 0.1 ml distilled water. |
Concentration | ~1.0 mg/ml (after reconstitution) |
Purification | Affinity Chromatography |
Conjugation | Unconjugated |
Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Predicted Protein Size | 136.5 kDa |
Gene Name | collagen type I alpha 1 |
Database Link | |
Background | Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. |
Synonyms | OI4 |
Reference Data | |
Protein Families | Druggable Genome |
Protein Pathways | ECM-receptor interaction, Focal adhesion |
Documents
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complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.