Cd8a Mouse Monoclonal Antibody [Clone ID: Rib 6/1]
Other products for "Cd8a"
Specifications
Product Data | |
Clone Name | Rib 6/1 |
Applications | FC, IHC |
Recommended Dilution | Immunohistochemistry on frozen sections (1 µg/ml; 1:200). Suggested positive control: rat spleen. Does not react on routinely processed paraffin sections. Flow cytometry (use 10 µl of neat antibody to label 10e6 cells). |
Reactivities | Rat |
Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Rat lymphoblasts |
Specificity | This antibody detcts T-cell subpopulation, thmocytes. Antigen distribution: Isolated cells: Subpopulation of T-cells in the peripheral blood. Tissue sections: Rib 6/1 detects the T-cell regions in lymphoid organs and stains cortical and medullary thymocytes positively. Monoclonal antibody Rib 6/1 is used for the identificaton of CD8 bearing cells, mainly Tcytotoxic and suppressor lymphocytes, in tissue sections and isolated cell suspensions. Preliminary results indicate that the Rib 6/1 antigen is the β-chain of the CD8 antigen which is not expressed by NK-cells. |
Formulation | Phosphate buffered saline pH 7.2 (PBS), 10 mg/ml bovine serum albumin (BSA) as a stabilizer and 0.01 % thimerosal as a preservative State: Purified State: Lyophilized Ig fraction |
Reconstitution Method | Restore with 0.5 ml distilled water. |
Purification | Affinity chromatography |
Conjugation | Unconjugated |
Storage | Store lyophilized at 2 - 8 °C and reconstituted at -20 °C. Avoid repeated freezing and thawing. |
Stability | Shelf life: One year from despatch. |
Gene Name | CD8a molecule |
Database Link | |
Background | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. |
Synonyms | CD8 alpha chain, CD8A, MAL |
Note | Cross reactivity with human monocytes has been observed in FACS. Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature 1. Wash in PBS 2. Block endogenous peroxidase 3. Wash in PBS 4. Block with 10% normal goat serum in PBS for 30 min. in a humid chamber 5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber 6. Wash in PBS 7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG (H+L) minimal-cross reaction to rat) for 1h in a humid chamber 8. Wash in PBS 9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer's hemalum |
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