CD11a (ITGAL) Mouse Monoclonal Antibody [Clone ID: MEM-25]

CAT#: BM4046LE

CD11a (ITGAL) mouse monoclonal antibody, clone MEM-25, Low Endotoxin

Specifications

Product Data

• Clone Name: MEM-25
• Applications: FC, FN, IP
• Recommended Dilution: Flow cytometry: 2 μg/ml.
  Immunoprecipitation: excellent antibody for immunoaffinity purification of LFA-1 complex.
  Functional application: Partially blocks binding of LFA-1 complex to ICAM-1.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Leukocytes from a pacient suffering from a LGL-type leukaemia
• Specificity: This antibody reacts with CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
• Formulation: Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized
  State: Low Endotoxin
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: integrin subunit alpha L
• Database Link:
  Entrez Gene 3683 Human
  UniProt ID P20701
• Background: CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
• Synonyms: Integrin alpha-L, LFA1, LFA-1