Desmoglein 2 (DSG2) Mouse Monoclonal Antibody [Clone ID: DG3.10]

CAT#: BM370

Desmoglein 2 (DSG2) mouse monoclonal antibody, clone DG3.10, Purified

Specifications

Product Data

• Clone Name: DG3.10
• Applications: IF, IHC, WB
• Recommended Dilution: Western blotting.
  Immunofluorescence Microscopy.
  Immunohistochemistry on Frozen Sections.
  Immunohistochemistry on Paraffin Embedded Tissue (after microwave treatment).
  Working Dilution: Dilute at least 1/10 with PBS, pH 7.4, for Immunohistochemical application.
  Incubation time: 1 h at RT, extended with paraffin.
• Reactivities: Bovine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: "Band 3" polypeptide of isolated desmosomes from Bovine muzzle epidermis
• Specificity: This antibody recognizes Desmoglein 1+2 (desmosome-specific cadherins) in desmosome-bearing cells. It does not react with cells containing plaque-bearing junctions of non-desmosomal type.
  Tumors Specifically Detected: All epithelium-derived tumors, notably carcinomas, and meningiomas.
  Polypeptide Reacting: Mr 165000 Desmoglein, "band 3" polypeptide of the desmosomal complex.
  Epitope Recognized: Localized at the repeating unit domain ("RUD") of the cytoplasmic C-terminal part of the polypeptide.
  Tested Reactivities on Cultured Cell Lines: A-431, MCF-7, TR-146, PLC and various other Human epithelial cell lines; Bovine BMGE+H, BMGE-H, MDBK.
• Formulation: Final Solution contains PBS, pH 7.4 with 0.09% Sodium Azide as preservative and 0.5% BSA as stabilizer
  State: Purified
  State: Lyophilized purified Ig fraction
• Reconstitution Method: Restore with 1.0 ml distilled water.
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: desmoglein 2
• Database Link:
  Entrez Gene 1829 Human
  UniProt ID Q14126
• Background: Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 and 2 are calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18.
• Synonyms: DSG1, DSG-1, CDHF4, Desmosomal glycoprotein 1, DG1, DGI, Pemphigus foliaceus antigen, Desmoglein1, DSG2, DSG-2, CDHF5, HDGC, Desmoglein2

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC)