MEKK1 (MAP3K1) pThr1402 Rabbit Polyclonal Antibody

CAT#: AP55761PU-S

MEKK1 (MAP3K1) pThr1402 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from JK cells (Lane 2), using MAP3K1 (Phospho-Thr1402) Antibody. The lane on the left is treated with antigen-specific peptide.

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  Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP3K1 (Phospho-Thr1402) Antibody (left)or the same antibody preincubated with blocking peptide (right).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1:500~1:1000.
  Immunohistochemistry on paraffin section: 1:50~1:100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of threonine 1402(T-G-A(p)-G-F) derived from Human MAP3K1 (KLH-conjugated)
• Specificity: The antibody detects endogenous levels of MAP3K1 only when phosphorylated at threonine 1402.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography using epitope-specific peptide
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 130 kDa
• Gene Name: mitogen-activated protein kinase kinase kinase 1
• Database Link:
  Entrez Gene 4214 Human
  UniProt ID Q13233
• Background: Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.
• Synonyms: MAPKKK1, MEKK, MEKK1, MAPK/ERK kinase kinase 1