HNRNPD pSer83 Rabbit Polyclonal Antibody

CAT#: AP55725PU-S

HNRNPD pSer83 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from JK cells (Lane 2), using hnRPD (Phospho-Ser83) Antibody. The lane on the left is treated with antigen-specific peptide.

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  Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using hnRPD (Phospho-Ser83) Antibody (left)or the same antibody preincubated with blocking peptide (right).

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  Immunofluorescence staining of methanol-fixed HeLa cells using hnRPD (Phospho-Ser83) Antibody.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1:500~1:1000.
  Immunohistochemistry on paraffin sections: 1:50~1:100.
  Immunofluorescence: 1:100~1:200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around phosphorylation site of Serine 83(N-S-S(p)-P-R) derived from Human hnRPD (KLH-conjugated)
• Specificity: The antibody detects endogenous levels of hnRPD only when phosphorylated at serine 83.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography using epitope-specific peptide
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 38 kDa
• Gene Name: heterogeneous nuclear ribonucleoprotein D
• Database Link:
  Entrez Gene 3184 Human
  UniProt ID Q14103
• Background: Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3' UTR of many proto-oncogenes and cytokine mRNAs. Also binds to double- and single-stranded DNA sequences in a specific manner and functions a transcription factor. Each of the RNA-binding domains specifically can bind solely to a single-stranded non-monotonous 5'-UUAG-3' sequence and also weaker to the single-stranded 5'-TTAGGG-3' telomeric DNA repeat. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
• Synonyms: AUF1, HNRPD, Heterogeneous nuclear ribonucleoprotein D0