MRPL50 (C-term) Rabbit Polyclonal Antibody

CAT#: AP53677PU-N

MRPL50 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of MRPL50 Antibody (C-term) in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the RM50 antibody detected the RM50 protein (arrow).

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  Formalin fixed, paraffin embedded human brain tissue stained with MRPL50 Antibody (C-term) followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of RM50 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1000
  Western Blot: 1/100-1/500.
  Immunohistochemistry on Paraffin Sections: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 118-147 amino acids from the C-terminal region of Human RM50.
• Specificity: This antibody recognizes Human MRPL50 (C-term).
• Formulation: PBS
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein A
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: mitochondrial ribosomal protein L50
• Database Link:
  Entrez Gene 54534 Human
  UniProt ID Q8N5N7
• Background:
  
  

  Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a putative 39S subunit protein and belongs to the L47P ribosomal protein family. Pseudogenes corresponding to this gene are found on chromosomes 2p, 2q, 5p, and 10q.

  
  
• Synonyms: L50mt, MRP-L50
• Note: Molecular Weight: 18325 Da