KDM3B (Center) Rabbit Polyclonal Antibody

CAT#: AP52266PU-N

KDM3B (Center) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of JHDM2b Antibody (Center) in Ramos cell line lysates (35ug/lane). This demonstrates the JHDM2b antibody detected the JHDM2b protein (arrow).

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  Flow cytometric analysis of Ramos cells using (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, WB
• Recommended Dilution: ELISA: 1/1000.
  Western Blot: 1/100-1/500.
  Flow Cytometry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 876-905 amino acids from the Central region of human JHDM2b
• Specificity: This antibody recognizes Human JMJD1B / KDM3B (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: lysine demethylase 3B
• Database Link:
  Entrez Gene 51780 Human
  UniProt ID Q7LBC6
• Background: Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.
• Synonyms: 5qNCA, C5orf7, JHDM2B, KIAA1082
• Note: Molecular Weight: 84kd Isoform 3. 191;581 Da