Interleukin-7 / IL7 Rabbit Polyclonal Antibody

CAT#: AP32852BT-S

Interleukin-7 / IL7 rabbit polyclonal antibody, Biotin

Product Images

Western Blot (Unreduced) usingIL7 antibody Cat.-No AP32852BT

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  Western Blot (Reduced) usingIL7 antibody Cat.-No AP32852BT

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Rat IL-7 by direct ELISA (using 100 ml/well) a concentration of approximately 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-7.
  Sandwich ELISA: To detect Rat IL-7 by sandwich ELISA (using 100 ml/well) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat IL-7 (Cat.-No AP32852PU) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-7.
  Western Blot: To detect Rat IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Rat IL-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure (>98%) E.coli derived recombinant Rat IL7 (Cat.-No PA266)
• Specificity: This antibody recognizes Rat IL7.
  Other species not tested.
• Formulation: PBS, pH 7.2
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  UniProt ID P56478
• Background: IL-7 is a hematopoietic growth factor which affects primarily early B and T cells. Produced by thymic stromal cells, spleen cells and keratinocytes, IL-7 can also co-stimulate the proliferation of mature T cells in combination with other factors such as ConA and IL-2. Human and murine IL-7 is cross-species reactive.
• Synonyms: IL-7