CD95 (FAS) Rabbit Polyclonal Antibody

CAT#: AP32048BT-N

CD95 (FAS) rabbit polyclonal antibody, Biotin

Product Images

Direct ELISA using sFas Receptor Antibody Cat.-No AP32048BT

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  Sandwich ELISA using sFas Receptor Antibody Cat.-No AP32048BT

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Human sFas Receptor by Direct ELISA (using 100 ml/well) a concentration of approximately 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody allows the detection of at least 0.2–0.4 ng/well of recombinant Human sFas Receptor.
  Sandwich ELISA: To detect Human sFas Receptor by Sandwich ELISA (using 100 ml/well) a concentration of 0.25–1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Anti-Human sFas Receptor (Cat.-No AP03248PU-N or AP03248PU-S) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of recombinant Human sFas Receptor.
  Western Blot: To detect Human sFas Receptor by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 mg/ml. When used in conjunction with compatible development reagents the detection limit for recombinant Human sFas Receptor is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly purified E.coli derived recombinant Human sFas Receptor (Cat.-No PA222)
• Specificity: This antibody recognizes Human sFas Receptor.
• Formulation: PBS, pH 7.2
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Centrifuge vial prior to opening.
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Fas cell surface death receptor
• Database Link:
  Entrez Gene 355 Human
  UniProt ID P25445
• Synonyms: FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6