Histone H3 Sheep Polyclonal Antibody
Specifications
Product Data | |
Applications | IF, IHC, IP, WB |
Recommended Dilution | Immunocytochemistry: 1/100. Immunohistochemistry on Paraffin Sections: 5 µg/ml. Immunoprecipitation. Western Blot: 1/300. |
Reactivities | Bovine, Human |
Host | Sheep |
Isotype | IgG |
Clonality | Polyclonal |
Immunogen | Intact Calf Histone H3 at 15.4 kD, 136 amino acids, complexed with RNA. |
Specificity | Recognizes Histone H3. Minimal cross-reactivity with linker Histone H1 and no cross-reactivity with Histone core proteins H2A, H2B, or H4. |
Formulation | PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction |
Concentration | lot specific |
Purification | Protein G Chromatography |
Conjugation | Unconjugated |
Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Background | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. |
Synonyms | H3 Histone family, HIST1H3 |
Reference Data |
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