c Kit (KIT) pTyr719 Rabbit Polyclonal Antibody

CAT#: AP26418AF-N

c Kit (KIT) pTyr719 rabbit polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA
• Recommended Dilution: ELISA: The antibody (approx. 1:64,000 diluted) can detect the above Tyr 719 phosphorylated c-kit peptide coated on ELISA plate.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide derived from tyrosine 719 phosphorylated c-kit, conjugated with KLH
• Specificity: This antibody reacts with Mouse, Human and other species with consensus c-kit peptide (SDSTNEYpMDMKPG).
• Formulation: 0.01M PBS, pH 7.4
  State: Azide Free
  State: Lyophilized Ig fraction
• Reconstitution Method: Restore with Double distilled water to adjust the final concentration to 1.00mg/ml.
• Purification: Protein G affinity
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 month or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: KIT proto-oncogene receptor tyrosine kinase
• Database Link:
  Entrez Gene 3815 Human
  UniProt ID P10721
• Background: c-kit, also known as CD117, is a receptor tyrosine kinase expressed at the surface of mast cells and certain types of stem cells. c-kit is activated and autophosphorylated upon binding with stem cell factor. Activated c-kit phosphorylates downstream SH2- domain-containing signalling molecules such as PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL. The binding is impaired when c-kit is mutated at tyrosine719. c-kit with phosphorylated tyrosine 719 binds to the p85 subunit of PI3 kinase in vitro and in vivo. C-kit is encoded by KIT gene and the gene is frequently over-expressed in gastrointestinal stromal tumor, myelogenous leukemia, and testicular germ cell tumor. Mutational loci of the gene are implicated with responsiveness to cancer drug imatinib.
• Synonyms: SCFR, KIT