Junctional Adhesion Molecule 1 (F11R) (2nd extracell. dom.) Rabbit Polyclonal Antibody

CAT#: AP26400PU-N

Junctional Adhesion Molecule 1 (F11R) (2nd extracell. dom.) rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: Flow cytometry: The typical starting working dilution is 1:50.
  Immunohistochemistry on paraffin sections: The typical starting working dilution is 1:50.
  Western blot: The typical starting working dilution is 1:50.
  Not useful for Immunoassays.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Extracellular domain of full-length human JAM-A
• Specificity: The antibody reacts with the 17 kDa extracellular domain 2 of the human JAM-A protein.
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% bovine serum albumin
  Preservative: 0.02% sodium azide
• Concentration: lot specific
• Purification: Protein A
• Conjugation: Unconjugated
• Storage:

  Store at 2 - 8 °C.
  

• Stability: Shelf life: one year from despatch.

• Gene Name: F11 receptor
• Database Link:
  Entrez Gene 50848 Human
  UniProt ID Q9Y624
• Background: Together with JAM-C (JAM-2) and JAM-B (VE-JAM or JAM-3), JAM-A belongs to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAMs are important for a variety of cellular processes, including tight junction assembly, leukocyte transmigration, platelet activation, angiogenesis and virus binding. JAM-A is expressed by endothelial and epithelial cells, platelets, neutrophils, monocytes, lymphocytes and erythrocytes. Like all other JAMs, JAM-A play an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-A also plays an important role in leukocyte transmigration. Leukocyte transmigration can be blocked by antibodies and by soluble JAM-A/Fc fusion proteins. Homophilic JAM-A interactions between leukocytes and the endothelium but also heterophilic interactions of JAM-A with the b2-integrin leukocyte function-associated antigen-1 (LFA-1) are considered to actively guide leukocytes during transmigration. Several studies imply a role of JAM-A in the initiation of atherosclerosis, since JAM-A is upregulated on early atherosclerotic endothelium and adhesion of activated platelets on activated endothelium is mediated by homophilic interactions of JAM-A.
• Synonyms: JAM-A, Platelet F11 receptor, F11R, JCAM, PAM1