Hap1 (C-term) Goat Polyclonal Antibody

CAT#: AP23773PU-N

Hap1 (C-term) goat polyclonal antibody, Aff - Purified

Product Images

HAP-1 antibody staining of Mouse Brain lysate at 0.1 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Peptide ELISA: Detection Limit: 1/32000.
  Western blot: 0.1-0.3µg/ml. Approx 100kDa band observed in Mouse and Rat lysates (calculated MW of 66.8kDa according to NP_034534.1).
• Reactivities: Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_034534.1.
• Specificity: Recognizes Mouse Hap1
• Formulation: Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: huntingtin-associated protein 1
• Database Link:
  Entrez Gene 15114 Mouse
  UniProt ID O35668
• Background: Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. It is a progressive disorder that generally begins in middle age with abnormalities of movement, cognition, personality and mood.
  HAP-1 is highly expressed in brain and shown to mediate the neuropathology of HD. The human protein interacts with huntingtin, with two cytoskeletal proteins (dynactin and pericentriolar autoantigen protein 1), and with a hepatocyte growth factor-regulated tyrosine kinase substrate. The interactions with cytoskeletal proteins and a kinase substrate suggest a role for this protein in vesicular trafficking or organelle transport. Two transcripts encoding different isoforms have been identified but one is a nonsense-mediated decay (NMD) candidate.
• Synonyms: Huntingtin-associated protein 1, HAP-1, HAP2, HLP1, Neuroan 1