GST3 (GSTP1) (C-term) Rabbit Polyclonal Antibody

CAT#: AP23405PU-N

GST3 (GSTP1) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis og Hela cell lysis using GSTπ antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: At 1-2μg/ml with the appropriate system to detect GSTpi in cells and tissues.
  Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect GSTpi in formalin fixed and paraffin embedded tissues. Boiling the sections is required.
  Immunocytochemistry: At 0.5-1μg/ml to detect GSTpi in acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A peptide mapping at the C-terminus of GSTpi of human origin
• Formulation: State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 1.2% sodium acetate or neutral PBS. If 0.5ml of PBS is used, the antibody concentration will be 100μg/ml.
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: glutathione S-transferase pi 1
• Database Link:
  Entrez Gene 14870 MouseEntrez Gene 24426 RatEntrez Gene 2950 Human
  UniProt ID P09211
• Background: Glutathione S-transferases pi, also known as GST3, present in all tissues and cells, with the exception of red cells, in which only erythrocyte GST (GSTe) is observed. The GST-pi gene has 7 exons and 6 introns contained within approximately 2.8 kilobases. The GST-pi gene is mapped to chromosome 11 .Placental glutathione-S-transferase-pi mRNA is abundantly expressed in human skin. GSTP does not contribute in vivo to the formation of glutathione conjugates of acetaminophen but plays a novel and unexpected role in the toxicity of this compound.
• Synonyms: Glutathione S-transferase P, GSTP1-1, GST pi, GST class-pi, FAEES3

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Drug metabolism - cytochrome P450, Glutathione metabolism, Metabolism of xenobiotics by cytochrome P450, Pathways in cancer, Prostate cancer