DOCK1 Rabbit Polyclonal Antibody

CAT#: AP21159PU-N

DOCK1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of DOCK 180 antibody (Cat.-No.: AP21159PU-N) in extracts from HUVEC cells.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of DOCK 180 protein.
  (region surrounding Lys1692)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 215 kDa
• Gene Name: dedicator of cytokinesis 1
• Database Link:
  Entrez Gene 330662 MouseEntrez Gene 1793 Human
  UniProt ID Q14185
• Background: The v-Crk oncogene product shares homologous amino acid sequences, designated Src homology region 2 (SH2) and SH3, with many molecules involved in signal transduction. The v-Crk cellular homolog, c-Crk, is a member of a newly emerging class of genes including Nck and GRB2/ASH which encode proteins that consist primarily of SH2 and SH3 domains. Two distinct human c-Crk cDNAs, designated Crk I and Crk II, have been identified and shown to represent alternative splice products of c-Crk. The major translational product of c-Crk I has been identified as a 28 kDa variably expressed protein, while c-Crk II encodes a widely expressed 40 kDa protein and a more variably expressed 42 kDa protein.
  The major c-Crk transforming activity appears associated with c-Crk I p28 expression. DOCK 180, a 180 kDa protein downstream of Crk, has been identified as a major Crk-associated protein. When DOCK 180 is recruited to the plasma membrane from a cytoplasmic reservoir, presumably by Crk, changes in cellular morphology and spindle formation occur, suggesting DOCK 180 to be a Crk effector molecule.
• Synonyms: ced5; DOCK180

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Focal adhesion, Regulation of actin cytoskeleton