HSF1 Rabbit Polyclonal Antibody

CAT#: AP21104PU-N

HSF1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of HSF1 antibody (Cat.-No.: AP21104PU-N) in extracts from K562 cells.

• 

  Immunohistochemistry (IHC) analyzes of HSF1 antibody (Cat.-No.: AP21104PU-N) in paraffin-embedded human colon carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of HSF1 protein.
  (region surrounding Asp135)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 57, 82 kDa
• Gene Name: heat shock transcription factor 1
• Database Link:
  Entrez Gene 15499 MouseEntrez Gene 3297 Human
  UniProt ID Q00613
• Background: Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development, and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway.
• Synonyms: HSTF1, HSF-1

Reference Data

• Protein Families: Transcription Factors