Granzyme K (GZMK) Rabbit Polyclonal Antibody

CAT#: AP20485PU-N

Granzyme K (GZMK) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Granzyme K antibody (Cat.-No AP20485PU-N) in extracts from Jurkat cells.

• 

  Immunohistochemistry analysis of Granzyme K (Cat.-No.: AP20485PU-N) in paraffin-embedded human lung carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Granzyme K protein.
  (region surrounding His85)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~33 kDa
• Gene Name: granzyme K
• Database Link:
  Entrez Gene 14945 MouseEntrez Gene 29165 RatEntrez Gene 3003 Human
  UniProt ID P49863
• Background: The granzyme family of proteins belong to the larger peptidase S1 family. Granzyme A and granzyme B are serine proteases that facilitate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Within the granules of activated CTLs, granzyme A and B are processed and converted to their active forms by the lysosomal cysteine protease cathepsin C. Once cleaved, these active proteases target distinct substrates for proteolysis and, thereby, mediate apoptosis through two different pathways. Granzyme H localizes to cytoplasmic granules of cytolytic T lymphocytes and is important for target cell lysis in cell-mediated immune responses. Granzyme K (GMZK), also designated granzyme-3 or NK-tryptase-2 (NK-TRYP-2), contains one peptidase S1 domain. Granzyme K is a serine protease localizing to the granules of natural killer cells and cytotoxic T lymphocytes. It is primarily expressed in thymus, lung, spleen and peripheral blood leukocytes.
• Synonyms: Granzyme-3, NK-tryptase-2, Fragmentin-3, TRYP2, NK-Tryp-2

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein, Transmembrane