CAMK2D (C-term) Rabbit Polyclonal Antibody

CAT#: AP13887PU-N

CAMK2D (C-term) rabbit polyclonal antibody, Purified

Product Images

The anti-CAMK2delta C-term Pab is used in Western blot to detect CAMK2 delta in serum-starved HeLa cell lysate.

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  Formalin-fixed and paraffin-embedded human cancer tissue (hepatocarcinoma) reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Formalin-fixed and paraffin-embedded human brain tissue reacted with CAMK2 delta Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blot: 1/100 -1/500.
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human, Monkey
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 448-478 amino acids from the C-terminal region of human CAMK2D (CAMK2 delta).
• Specificity: This antibody detects CAMK2D at C-term.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: calcium/calmodulin dependent protein kinase II delta
• Database Link:
  Entrez Gene 817 Human
  UniProt ID Q13557
• Background: CaM-kinase II (CAMK2) is a prominent Ser/Thr protein kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Likely autophosphorylation of Thr-286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state. CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Expression of CAMK2 delta is significantly increased in patients suffering from dilated cardiomyopathy.
• Synonyms: CAMKD, CaM-kinase II delta chain, CaM kinase II subunit delta, CaMK-II subunit delta
• Note: Molecular weight: 56297 Da