Parp12 (C-term) Rabbit Polyclonal Antibody

CAT#: AP13306PU-N

Parp12 (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of Polyclonal anti-Parp12 antibody in mouse muscle tissue lysates (35ug/lane). Parp12 (arrow) was detected using the purified Parp12 antibody at 1/60 dilution.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/50 - 1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This PARP12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of mouse PARP12.
• Specificity: This antibody reacts to PARP12.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: poly (ADP-ribose) polymerase family, member 12
• Database Link:
  UniProt ID Q8BZ20
• Background: Poly(ADP-ribosyl)ation is an immediate DNA-damage-dependent post-translational modification of histones and other nuclear proteins that contributes to the survival of injured proliferating cells. Poly(ADP-ribose) polymerases (PARPs) now constitute a large family of 18 proteins, encoded by different genes and displaying a conserved catalytic domain in which PARP-1 (113 kDa), the founding member, and PARP-2 (62 kDa) are so far the sole enzymes whose catalytic activity has been shown to be immediately stimulated by DNA strand breaks. A large repertoire of sequences encoding novel PARPs now extends considerably the field of poly(ADP-ribosyl)ation reactions to various aspects of the cell biology including cell proliferation and cell death. Some of these new members interact with each other, share common partners and common subcellular localizations suggesting possible fine tuning in the regulation of this post-translational modification of proteins.
• Synonyms: PARP-12, ZC3HDC1