MEKK1 (MAP3K1) pThr1383 Rabbit Polyclonal Antibody

CAT#: AP12773PU-N

MEKK1 (MAP3K1) pThr1383 rabbit polyclonal antibody, Aff - Purified

Product Images

Dot blot analysis of Phospho-MAP3K1-Thr1383 Pab (Cat#AP12773PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration were 0.5ug per ml.

Specifications

Product Data

• Recommended Dilution: ELISA: 1/1,000.
  Dot Blot: 1/100-1/500.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T1383 of human MAP3K1.
• Specificity: This antibody detects MAP3K1 pThr1383.
  Predicted to cross react with Mouse (100% Antigen Homology).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: mitogen-activated protein kinase kinase kinase 1
• Database Link:
  Entrez Gene 4214 Human
  UniProt ID Q13233
• Background: Component of a protein kinase signal transduction cascade. Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4. Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway.
• Synonyms: MAPKKK1, MEKK, MEKK1, MAPK/ERK kinase kinase 1
• Note: Molecular weight: 164340 Da