MET pTyr1349 Rabbit Polyclonal Antibody

CAT#: AP12647PU-N

MET pTyr1349 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis using anti-MET pTyr1349 Pab to detect MET-Y1349 in mouse kidney tissue lysate.

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/1000
  Immunohistochemistry: 1/50~1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a 4=4 Chain MAPS conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1349 of human MET. Synthetic phosphopeptide was selected from region 1345~1357 of human MET.
• Specificity: This antibody detects HGF Receptor (MET) pTyr1349.
  Predicted to cross react with Mouse and Rabbit (100% Antigen Homology).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: MET proto-oncogene, receptor tyrosine kinase
• Database Link:
  Entrez Gene 4233 Human
  UniProt ID P08581
• Background: The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma.
• Synonyms: Hepatocyte growth factor receptor, MET, Scatter factor receptor, HGF/SF receptor, c-Met
• Note: Molecular weight: 155527 Da