ARFGAP1 (C-term) Rabbit Polyclonal Antibody

CAT#: AP12128PU-N

ARFGAP1 (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of ARFGrified Pab. Data courtesy of Dr. Dan Cassel, Department of Biology, Technion, Haifa, Israel.

• 

  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human, Rat
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ARFGAP1.
• Specificity: This antibody is specific to ARFGAP1 (C-term).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: ADP ribosylation factor GTPase activating protein 1
• Database Link:
  Entrez Gene 55738 Human
  UniProt ID Q8N6T3
• Background: ARFGAP1 is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine.
• Synonyms: ARF1GAP
• Note: Predicted Molecular weight: 44668 Da