ALDH6A1 (N-term) Rabbit Polyclonal Antibody

CAT#: AP11461PU-N

ALDH6A1 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of ALDH6A1 Antibody (N-term) in T47D cell line lysates (35 ug/lane). ALDH6A1 (arrow) was detected using the purified Pab (1:60 dilution).

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  Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with ALDH6A1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA 1:1,000.
  Western blot 1:50 - 1:100.
  Immunohistochemistry 1:10 - 1:50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide selected from the N-terminal region of human ALDH6A1
• Specificity: This antibody detects ALDH6A1 at N-term.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: aldehyde dehydrogenase 6 family member A1
• Database Link:
  Entrez Gene 4329 Human
  UniProt ID Q02252
• Background: ALDH6A1 belongs to the aldehyde dehydrogenases family of proteins. This enzyme plays a role in the valine and pyrimidine catabolic pathways. This protein is a mitochondrial methylmalonate semialdehyde dehydrogenase, and catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.
• Synonyms: MGC40271; MMSADHA; MMSDH
• Note: Molecular weight: 57858 Da