MDM2 Rabbit Polyclonal Antibody

CAT#: AP11259PU-N

MDM2 rabbit polyclonal antibody, Aff - Purified

Product Images

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with the MDM2 Antibody (S166), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: ELISA: 1/1,000.
  Immunohistochemistry: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S166 of human MDM2.
• Specificity: This antibody is specific to MDM2.
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein A Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: MDM2 proto-oncogene
• Database Link:
  Entrez Gene 4193 Human
  UniProt ID Q00987
• Background: MDM2 is a target of the transcription factor tumor protein p53. This protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.
• Synonyms: p53-binding protein Mdm2, Oncoprotein Mdm2, Double minute 2 protein, Hdm2
• Note: Predicted MW: 55232 Da