Sumo 3 (SUMO3) (Center) Rabbit Polyclonal Antibody

CAT#: AP11222PU-N

Sumo 3 (SUMO3) (Center) rabbit polyclonal antibody, Purified

Product Images

(LEFT) Western Blot analysis using anti-SUMO2/3 Pab to detect SUMO2/3 in Saos-2 cell lysate. (RIGHT) Western blot analysis of SUMO3 (arrow) using rabbit polyclonal SUMO2/3 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SUMO3 gene (Lane 2) (Origene Technologies).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blot: 1/100-1/500.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide at the center region of human SUMO2/3.
• Specificity: This antibody is specific to SUMO2/3 (Center).
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: small ubiquitin-like modifier 3
• Database Link:
  Entrez Gene 6612 Human
  UniProt ID P55854
• Background: SUMO2 and SUMO3 are members of the SUMO (small ubiquitin-like modifier) protein family. This protein family functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. In vertebrates, three members of the SUMO family have been described, SUMO 1 and the functionally distinct homologues SUMO 2 and SUMO 3. SUMO modification sites present in the N terminal regions of SUMO 2 and SUMO 3 are utilized by SAE1/SAE2 (SUMO E1) and Ubc9 (SUMO E2) to form polymeric chains of SUMO 2 and SUMO 3 on protein substrates, a property not shared by SUMO 1.
• Synonyms: SUMO-2, SMT3B, SMT3H2, HSMT3, Sentrin-2, SUMO-3, SMT3 homolog 1, SMT3H1
• Note: Predicted MW: 10.871 kDa